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With your Low E string losing its tune I Would be looking at your tem. getting its set up correctly may help with the guitar holding it tune.
Fender Strat setup for a Foating trem:-
If you (like me) Use a foating setup.
You will need to
Put some blokes in the tem to hold it where you want it.
then wind the screws on the back sping plate up hard.
(this will stop the tem from moving while you tune the strings)
then tune and stetch the strings in (and play them a bit) and tune them again.
then remove the tem blocks and unwind the back srew plate, til the string s go back in tune.
then you will have set the fender floating trem. as per leo's design.
this should fix your detuning issue with the Low E.
Cheers
AC
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